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custom-made cryo laser apparatus  (Design Solutions Inc)
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Design Solutions Inc custom-made cryo laser apparatus
Production, cryopreservation, and recovery of single-polyp microfragments (SPMFs) from the coral Porites compressa . Corals were cut into microfragments and grown on plastic sheets, then individual polyps were excised from thin primary growth areas using a biopsy punch and allowed to heal in an incubator to form single-polyp microfragments (SPMFs). Single-polyp microfragments were pre-incubated in 0.07 mg/mL (4.5 × 10 np/m -3 ) 1064 nm resonant PEG-coated gold nanorods in FSW for 1 hour, then equilibrated with cryoprotectant solution in three steps over 4 minutes on the cryotop. The cryotop with SPMF was transferred to the <t>cryo</t> device <t>for</t> <t>vitrification</t> and laser nanowarming using an iWeld 980 Series, 60-joule, Nd:YAG unpolarised infrared laser (240 V, 9.0 ms, ∼ 8.2 J). Single-polyp microfragments were then rehydrated in five steps over 4 minutes, followed by recovery in a 24-well plate overnight in an incubator without light.
Custom Made Cryo Laser Apparatus, supplied by Design Solutions Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom-made cryo laser apparatus/product/Design Solutions Inc
Average 90 stars, based on 1 article reviews
custom-made cryo laser apparatus - by Bioz Stars, 2026-04
90/100 stars
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Production, cryopreservation, and recovery of single-polyp microfragments (SPMFs) from the coral Porites compressa . Corals were cut into microfragments and grown on plastic sheets, then individual polyps were excised from thin primary growth areas using a biopsy punch and allowed to heal in an incubator to form single-polyp microfragments (SPMFs). Single-polyp microfragments were pre-incubated in 0.07 mg/mL (4.5 × 10 np/m -3 ) 1064 nm resonant PEG-coated gold nanorods in FSW for 1 hour, then equilibrated with cryoprotectant solution in three steps over 4 minutes on the cryotop. The cryotop with SPMF was transferred to the cryo device for vitrification and laser nanowarming using an iWeld 980 Series, 60-joule, Nd:YAG unpolarised infrared laser (240 V, 9.0 ms, ∼ 8.2 J). Single-polyp microfragments were then rehydrated in five steps over 4 minutes, followed by recovery in a 24-well plate overnight in an incubator without light.

Journal: bioRxiv

Article Title: The first proof of concept demonstration of nanowarming in coral tissue

doi: 10.1101/2023.03.16.533048

Figure Lengend Snippet: Production, cryopreservation, and recovery of single-polyp microfragments (SPMFs) from the coral Porites compressa . Corals were cut into microfragments and grown on plastic sheets, then individual polyps were excised from thin primary growth areas using a biopsy punch and allowed to heal in an incubator to form single-polyp microfragments (SPMFs). Single-polyp microfragments were pre-incubated in 0.07 mg/mL (4.5 × 10 np/m -3 ) 1064 nm resonant PEG-coated gold nanorods in FSW for 1 hour, then equilibrated with cryoprotectant solution in three steps over 4 minutes on the cryotop. The cryotop with SPMF was transferred to the cryo device for vitrification and laser nanowarming using an iWeld 980 Series, 60-joule, Nd:YAG unpolarised infrared laser (240 V, 9.0 ms, ∼ 8.2 J). Single-polyp microfragments were then rehydrated in five steps over 4 minutes, followed by recovery in a 24-well plate overnight in an incubator without light.

Article Snippet: The wooden holder with the cryo blade and SPMF was then transferred into the chamber of a bench-top iWeld 980 Series, 60 joule, Nd:YAG unpolarised infrared laser (model number 585-986-080; LaserStar Technologies Corporation, Orlando, FL, USA) and inserted into the arm of a custom-made cryo laser apparatus (Design Solutions, Inc., Chanhassen, MN, USA) for vitrification and laser nanowarming as described in Daly et al. . After 1 min exposure to the full-strength vitrification solution, the cryo apparatus was used to lower the sample into a liquid nitrogen bath for vitrification.

Techniques: Incubation